The difference between the internal and externally rotated cryovials is their bottom. The internal rotation can clearly see the bottom of the tube, while the external rotation is blocked by the circular bottom with a gear shape, so the bottom surface cannot be seen clearly. This difference is very important when the cryopreservation solution is aspirated after the cells are recovered and centrifuged. The inner rotation can easily see that the liquid is completely sucked away without the cells being sucked out. The outer rotation of the cryopreservation tube is Cells may be aspirated. There should be no effect on cryopreservation.
If it is in a -80°C refrigerator, both internal and external rotations can be used, but internal rotation should be used in the liquid phase of liquid nitrogen. Although both internal rotation and external rotation will enter liquid nitrogen, the probability of an internal rotation explosion is small. Generally speaking, the gas phase does not matter, and it is relatively safe to use internal rotation.
Compared with the external rotation, the volume of the internal rotation cryotube needs to be turned slightly less, but under the ultra-low temperature conditions of liquid nitrogen, it can prevent the liquid nitrogen from entering the cryopreservation tube through the thread due to inconsistent shrinkage of the cryopreservation tube. Therefore, internal rotation is generally used for cryopreservation in liquid nitrogen. In other cases, the requirements are not so strict.
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