The micropipette is a common and very important chemical laboratory instrument used to transfer liquid volumes in the microliter range with precision and accuracy. Micropipettes are available in single and multi-channel configurations. Multichannel micropipettes are ideal for ELISA (diagnostic tests) as well as molecular screening studies of kinetics and DNA amplification. Single-channel micropipettes are used in chemistry laboratories conducting in-depth research in microbiology, molecular biology, cell culture, immunology, biochemistry, analytical chemistry, genomic research, and genetics.
Components of a micropipette
Micropipettes come in many shapes and sizes. All micropipettes share certain characteristics. Plungers, digital displays, and tip cones, as well as tip ejectors and grips, are part of the package. Some micropipettes include calibration tools as well as micropipette stands as options.
Plunger: The plunger is the very top of the micropipette and is used to expel and collect the desired amount of liquid into the tip of the micropipette.
Eject button: It is used to eject the tip of the micropipette without touching it. This button is attached to the gauge strip and pushes the micropipette tip away.
Volume adjustment: Rotate a circle at the top of the micropipette to decrease and increase the set volume. The required intake is ensured by adjusting the volume using a volume adjustment.
Volume window: The volume window displays the adjusted volume. Within the range of this pipette, different liquid values can also be set. Different micropipettes allow different volume ranges to be measured.
Pipette: A pipette is a tube that contains air and holds a pipette tip. Pushing the plunger forces a volume of air out of the barrel, and releasing the plunger allows the air to return. The plunger has two stops for aspirating liquid in forward and reverse pipetting.
Pipette Tips: These attach to micropipettes and are used to aspirate liquids and then transfer them from one location to another. Different-sized tips are used to aspirate different volumes of liquid.
Types of Micropipettes
Air displacement micropipettes:
The air displacement principle used by micropipettes consists of a piston that can aspirate and disperse a liquid sample while moving an air pocket up, down, and back. The internal mechanism of the pipette is not in direct contact with the liquid or sample. To pull the liquid in and out of the pipette, irreplaceable pipette tips are used.
Positive displacement micropipettes:
The plunger in these micropipettes is in direct contact with the sample or material. The disposable tip of a positive displacement micropipette is an extremely small syringe consisting of a capillary and a piston (movable inside) that moves liquid directly.
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