How To Use And Handle 1.0ml Cryogenic Tubes Correctly
Proper handling and use of 1.0ml cryogenic tubes
are important to ensure the integrity and longevity of the samples stored within them, following any additional instructions provided by the cryogenic tube manufacturer.
- Label the tubes: Before storing samples, it is important to label each tube with the necessary information such as sample name, date, and any other relevant details.
- Prepare samples: Prepare samples according to appropriate protocols, taking care to avoid contamination.
Fill tube: Fill the cryogenic tube with the appropriate amount of sample, leaving some headspace at the top of the tube to allow for expansion during freezing.
- Cap tubes: Cap tubes tightly to prevent any leakage or contamination.
- Freezing the tubes: Place the tubes in a speed-controlled freezer or container of isopropanol and freeze the samples at a rate of 1°C per minute until the desired temperature is reached.
- Storage of test tubes: After the samples are frozen, transfer the test tubes to a liquid nitrogen refrigerator or a -80°C freezer for long-term storage.
-Thaw the tubes: When samples need to be used, remove the tubes from the refrigerator and place them in a water bath or under running water to quickly thaw. Do not allow samples to thaw at room temperature.
In addition to these steps, it is also important to handle the cryogenic tubes carefully to avoid any damage or breakage. Always use proper personal protective equipment, such as gloves, and avoid dropping or mishandling tubes.
How Big Is The Capacity Of The Cryogenic Tubes To Meet The Needs Of The Experiment
The volume of cryogenic storage vials
adequate for an experiment may vary depending on the type and amount of samples stored. Here are some factors to consider:
- Sample volume: The volume of the cryogenic storage vial should be sufficient to accommodate the volume of the stored sample, with some headspace for expansion during freezing.
- Sample type: Different sample types may require different amounts of storage. For example, cell culture may require larger volumes than protein purification.
- Sampling Frequency: If you store samples frequently, you may want to consider the volume and number of vials needed to meet your experimental needs.
- Storage Space: Consider the amount of storage space available for cryogenic vials in the laboratory.
Common cryogenic storage vials range in size from 0.5ml to 5ml, with 1.8ml and 2ml vials most commonly used. However, the exact capacity required will depend on the factors mentioned above.